| 產品名 | 脫氧核糖核酸酶Ⅰ（牛胰） |
| 別名 | 去氧核糖核酸酶;脫氧核糖核酸; 脫氧核糖核酸酶 ;胰道酶; 胰克氧核糖核酸酶; 除氧細胞核酸酶(牛胰); 去氧細胞核酸酶 |
| 英文名 | Deoxyribonuclease |
| CAS號 | 9003-98-9 |
| 分子式 | C12H22N4O6 |
| 分子量 | 318.32628 |
| EINECS編號 | 232-667-0 |
| MDL號 |
https://onlinelibrary.wiley.com/doi/10.1002/adfm.202315275
【穩定性 Stability】
最佳的工作范圍是 pH 7-8，DNase 的活性依賴于 Ca2+， 并可被二價金屬離子如 Mn2+，Zn2+等激活。5mM Ca2+ 可保護酶使其不被水解，而 0.1mM Ca2+可使酶失活速 率降至原來的 1/2。
The optimal working range is pH 7-8, and the activity of DNase depends on Ca2+ and can be activated by divalent metal ions such as Mn2+, Zn2+, etc. 5mM Ca2+ can protect the enzyme from hydrolysis, while 0.1mM Ca2+ can reduce the enzyme inactivation rate to half.
【單位定義 Definition of unit】
以 1.5ml(0.15mg/ml 小牛胸腺 DNA)作底物,在 35℃且 pH5.0 的條件下，1 分鐘內使反應液在波長 260nm 處的 吸光度增加 0.001 所需的酶量。（一個酶活力單位以 U 表示）
Using 1.5ml (0.15mg/ml calf thymus DNA) as substrate, the required enzyme amount is required to increase the absorbance of the reaction solution by 0.001 at a wavelength of 260nm within 1 minute under the condition of 35 ℃ and pH 5.0. (One enzyme activity unit is represented by U)
【產品特性 Properties】
1、 Mg2+存在條件下，DNase 可隨機識別和切斷 DNA 任一條鏈上的任意位點；而在 Mn2+存在條件下， DNase 識別 DNA 的兩條鏈并在幾乎相同的位點進 行切割；
Under the presence of Mg2+, DNase can randomly recognize and cleave any site on any strand of DNA; In the presence of Mn2+, DNase recognizes two strands of DNA and cleaves them at almost identical sites;
2、 DNase 可水解多種形式 DNA，如單鏈 DNA、雙鏈 DNA，甚至染色質（其切割速率受組蛋白影響）。 DNase can hydrolyze various forms of DNA, such as single stranded DNA, double stranded DNA, and even chromatin (its cleavage rate is influenced by histones).
COA搜索：
客服一
13813788405